Reverse Complement Tool
Reverse Complement Tool
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DNA sequence converter
December 2024© Sebastian F. Carvello, Imperial College LondonORCID iD: 0009-0003-9863-123X | Google Scholar | ResearchGateCorrespondence: sf@carvello.org / sebastian.carvello23@imperial.ac.uk
The complementary sequence is formed by replacing each nucleotide with its Watson-Crick complement. The reverse sequence is simply the original sequence read backwards. The reverse-complement is obtained by first finding the complement of the sequence and then reversing it, representing the complementary strand's sequence read in the same direction (e.g. 5' to 3').
Reverse
Complement
Reverse-complement
Note: By default, the tool formats the output sequence into lines of 60 characters each, which is a common practice for readability, especially in FASTA files. To obtain the output as a single line string, use the 'Copy without Line Breaks' button (the FASTA header will be preserved).
How to cite this tool:
Carvello, S.F. (2024) Reverse Complement Tool – DNA sequence converter. Available at: https://ug.sebc.me/labs/reverse-complement.
I'm always interested to hear from researchers and educators putting these tools to good use, so please do feel free to drop me a line if you've found this converter helpful or cited it — sf@carvello.org :)
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